EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, Keasling JD, Borodina I
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73271 | pCfB2899(X-2 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980) |
| 73272 | pCfB3034(X-3 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744) |
| 73273 | pCfB3035(X-4 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310) |
| 73274 | pCfB3036(XI-1 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573) |
| 73275 | pCfB2903(XI-2 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913) |
| 73276 | pCfB2904(XI-3 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567) |
| 73277 | pCfB3037(XI-5 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967) |
| 73278 | pCfB3038(XII-1 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720) |
| 73279 | pCfB3039(XII-2 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939) |
| 73280 | pCfB3040(XII-4 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248) |
| 73281 | pCfB2909(XII-5 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357) |
| 73282 | pCfB3020(gRNA X-2) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2 |
| 73283 | pCfB3041(gRNA X-3) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3 |
| 73284 | pCfB3042(gRNA X-4) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2 |
| 73285 | pCfB3043(gRNA XI-1) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1 |
| 73286 | pCfB3044(gRNA XI-2) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2 |
| 73287 | pCfB3045(gRNA XI-3) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3 |
| 73288 | pCfB3046(gRNA XI-5) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5 |
| 73289 | pCfB3047(gRNA XII-1) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1 |
| 73290 | pCfB3048(gRNA XII-2) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2 |
| 73291 | pCfB3049(gRNA XII-4) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4 |
| 73292 | pCfB3050(gRNA XII-5) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5 |
| 73293 | pCfB3051(gRNA X-3 XI-2 XII-2) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2 |
| 73294 | pCfB3052(gRNA X-4, XI-3, XII-5) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5 |
| 73295 | pCfB3053(gRNA X-2, XI-5, XII-4) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4 |
| 73296 | pCfB1975 (pUC19-PTEF1-PPGK1) | Example plasmid containing a bidirectional promoter |
| 78230 | p1977 (pUC19-PTDH3-PTEF1) | Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1 |
| 78231 | pCfB2312 (TEF1p-Cas9-CYC1t_kanMX) | Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance |
| 78232 | pTAJAK-71 (pESC-NatMXsyn-USER) | Empty vector, for the insertion of gRNA expression cassettes into the vector. |