pGEX-4T-1-XPLN
(Plasmid #73375)

• Purpose: GST-tagged expression of XPLN (ARHGEF3) in bacteria
• Depositing Labs: Jie Chen, Krister Wennerberg
• Publication: Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72. Epub 2002 Sep 6.

Backbone

• Vector backbone: pGEX 4T-1
• Backbone manufacturer: Amersham Biosciences
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: arhgef3
• Alt name: XPLN
• Species: H. sapiens (human)
• Entrez Gene: ARHGEF3 (a.k.a. GEF3, STA3, XPLN)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid was received from from Professor Krister Wennerberg (University of Helsinki, Helsinki).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original Publication: XPLN, a Guanine Nucleotide Exchange Factor for RhoA and RhoB, But Not RhoC