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251 pTol2Dest
(Plasmid #73484)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73484 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTol2005b

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    attR1/attR2 cassette
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site PspOMI (not destroyed)
  • 5′ sequencing primer M13-FP
  • 3′ sequencing primer M13R-pUC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    251 pTol2Dest was a gift from Nathan Lawson (Addgene plasmid # 73484 ; http://n2t.net/addgene:73484 ; RRID:Addgene_73484)
  • For your References section:

    Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311