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CMV-CAD
(Plasmid #73567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73567 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 73567-D50 50 μg of DNA in Tris buffer $495
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DNA 73567-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGCP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3980
  • Total vector size (bp) 4307
  • Modifications to backbone
    The EGFP gene was deleted and replaced with the CAD gene (STIM1 342-448).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAD (STIM1 342-448)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    327
  • GenBank ID
    NM_003156
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Promoter CMV Immediate Early

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TGACGTCAATGGGAGTTTG
  • 3′ sequencing primer ACATTGATGAGTTTGGAC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CAD corresponds to amino acids 342-448 of human STIM1.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-CAD was a gift from Michael Cahalan (Addgene plasmid # 73567 ; http://n2t.net/addgene:73567 ; RRID:Addgene_73567)

  • For your References section:

    Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Dynes JL, Amcheslavsky A, Cahalan MD. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 10.1073/pnas.1523410113. Epub 2015 Dec 28. 10.1073/pnas.1523410113 PubMed 26712003
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