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pSIREN-RetroQ-shN
(Plasmid #73665)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73665 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pSIREN RetroQ
  • Backbone manufacturer
    Clonetech
  • Modifications to backbone
    None
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA Neg Control
  • gRNA/shRNA sequence
    Control Sequence
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer U6 Promoter
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIREN-RetroQ-shN was a gift from Joe Landry (Addgene plasmid # 73665 ; http://n2t.net/addgene:73665 ; RRID:Addgene_73665)