|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25789||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameshRNA against human CDK6
Alt nameshRNA CDK6
SpeciesH. sapiens (human)
Insert Size (bp)53
Entrez GeneCDK6 (a.k.a. MCPH12, PLSTIRE)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GGGCAGGAAGAGGGCCTAT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSIREN-RetroQ-CDK6-shRNA was a gift from Judy Lieberman (Addgene plasmid # 25789 ; http://n2t.net/addgene:25789 ; RRID:Addgene_25789)
For your References section:miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, Lieberman J. Blood. 2009 Sep 3. 114(10):2181-92. 10.1182/blood-2009-02-205062 PubMed 19584398