PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73806||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter T7
/ Fusion Protein
- his-tag to facilitate purification (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that this plasmid expresses only Peredox, with no mCherry or mCitrine to use for ratio measurements. It is best used for fluorescence lifetime measurements.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSetB-Peredox was a gift from Gary Yellen (Addgene plasmid # 73806 ; http://n2t.net/addgene:73806 ; RRID:Addgene_73806)
For your References section:Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging. Mongeon R, Venkatachalam V, Yellen G. Antioxid Redox Signal. 2016 Mar 18. 10.1089/ars.2015.6593 PubMed 26857245