|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73841||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDictybase (http://dictybase.org/)
- Backbone size w/o insert (bp) 6213
- Total vector size (bp) 9597
Vector typeDictyostelium discoideum expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsWhen expressing in Dictyostelium discoideum, the depositing laboratory recommends strain AX4. For dictyostelium, growth must be at 21-23 degrees Celsius.
Gene/Insert namePhyB(1-917) with C-term his tag
SpeciesA. thaliana (mustard weed)
Insert Size (bp)2784
Entrez GenePIL2 (a.k.a. AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2)
/ Fusion Protein
- 6x his tag (C terminal on insert)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEG03 was a gift from Junghae Suh & Jeffrey Tabor (Addgene plasmid # 73841 ; http://n2t.net/addgene:73841 ; RRID:Addgene_73841)
For your References section:Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. Gomez EJ, Gerhardt K, Judd J, Tabor JJ, Suh J. ACS Nano. 2015 Nov 30. 10.1021/acsnano.5b05558 PubMed 26618393