|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74121||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerProduced in Jing-Ke Weng lab
- Backbone size w/o insert (bp) 5350
- Total vector size (bp) 6456
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namePhotinus pyralis firefly luciferin sulfotransferase
Insert Size (bp)1113
- Promoter T7
/ Fusion Protein
- 8x-His, TEV protease site (ENLYFQ) (N terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Expressed robustly in E.coli. Catalyzes sulfonation / desulfonation of firefly luciferin / sulfoluciferin with PAPS / PAP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJKW 0643 was a gift from Jing-Ke Weng (Addgene plasmid # 74121 ; http://n2t.net/addgene:74121 ; RRID:Addgene_74121)
For your References section:Sulfoluciferin is biosynthesized by a specialized luciferin sulfotransferase in fireflies. Fallon TR, Li FS, Vicent MA, Weng JK. Biochemistry. 2016 May 26. 10.1021/acs.biochem.6b00402 PubMed 27227579