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pJKW 0643
(Plasmid #74121)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74121 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHis8-4
  • Backbone manufacturer
    Produced in Jing-Ke Weng lab
  • Backbone size w/o insert (bp) 5350
  • Total vector size (bp) 6456
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Photinus pyralis firefly luciferin sulfotransferase
  • Alt name
    LST
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1113
  • GenBank ID
    KX097998
  • Promoter T7
  • Tag / Fusion Protein
    • 8x-His, TEV protease site (ENLYFQ) (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Expressed robustly in E.coli. Catalyzes sulfonation / desulfonation of firefly luciferin / sulfoluciferin with PAPS / PAP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJKW 0643 was a gift from Jing-Ke Weng (Addgene plasmid # 74121)
  • For your References section:

    Sulfoluciferin is biosynthesized by a specialized luciferin sulfotransferase in fireflies. Fallon TR, Li FS, Vicent MA, Weng JK. Biochemistry. 2016 May 26. 10.1021/acs.biochem.6b00402 PubMed 27227579