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pCMV-myc-FRNK
(Plasmid #74481)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74481 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-myc
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRNK
  • Species
    G. gallus (chicken)
  • Mutation
    FRNK is the C-terminal, noncatalytic domain of the FAK kinase
  • Entrez Gene
    PTK2 (a.k.a. FAK, FAK1, FRNK, p125FAK)
  • Promoter CMV
  • Tag / Fusion Protein
    • c-myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-myc-FRNK was a gift from J Thomas Parsons (Addgene plasmid # 74481 ; http://n2t.net/addgene:74481 ; RRID:Addgene_74481)
  • For your References section:

    Induction of apoptosis after expression of PYK2, a tyrosine kinase structurally related to focal adhesion kinase. Xiong W, Parsons JT. J Cell Biol. 1997 Oct 20;139(2):529-39. PubMed 9334354