PurposeRetroviral expression of FYN
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74510||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneFYN (a.k.a. SLK, SYN, p59-FYN)
- Promoter unknown
/ Fusion Proteins
- Myc (C terminal on backbone)
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer MSCV_C_rev2 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The depositor notes that a BamHI restriction site was inserted following the start codon of FYN. This site does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCVneo-FYN-WT-Myc-FLAG was a gift from Lars Rönnstrand (Addgene plasmid # 74510 ; http://n2t.net/addgene:74510 ; RRID:Addgene_74510)
For your References section:FYN expression potentiates FLT3-ITD induced STAT5 signaling in acute myeloid leukemia. Chougule RA, Kazi JU, Ronnstrand L. Oncotarget. 2016 Feb 2. doi: 10.18632/oncotarget.7128. 10.18632/oncotarget.7128 PubMed 26848862