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pIM690
(Plasmid #74638)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74638 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFM689
  • Backbone manufacturer
    Marian Farkasovsky
  • Backbone size w/o insert (bp) 4905
  • Total vector size (bp) 4677
  • Modifications to backbone
    Replacement of mCherry with yTagRFP-T
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yTagRFP-T

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    M. Whiteway, McGill University, Montreal, Canada

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compared to the published sequence of the pFA6a-hphNT1 backbone vector (Janke, et al., 2004, Yeast 21: 947-962) we found one PstI site missing in its sequence. It is the site in the vector –promoter part and there is a substitution of A by G in position 284 bp that arose most probably during construction of preceding cassettes, e.g. pFA6-hphMX4. We introduced the substitution also into maps of our modules carrying the hygromycin resistance - pFM689, pIM690 and pIM691.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIM690 was a gift from Jiří Hašek (Addgene plasmid # 74638 ; http://n2t.net/addgene:74638 ; RRID:Addgene_74638)
  • For your References section:

    New integrative modules for multicolor-protein labeling and live-cell imaging in Saccharomyces cerevisiae. Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J. FEMS Yeast Res. 2016 Mar 17. pii: fow027. 10.1093/femsyr/fow027 PubMed 26994102