-
Purposeretroviral expression of JARID1C
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneMSCV
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameJARID1C
-
SpeciesH. sapiens (human)
-
Entrez GeneKDM5C (a.k.a. DXS1272E, JARID1C, MRX13, MRXJ, MRXSCJ, MRXSJ, SMCX, XE169)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP is driven by a separate promoter (UBC promoter), and is used only to track cells and the efficiency of infection/transfection.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSCV-dGFP-JARID1C was a gift from Giovanni Tonon (Addgene plasmid # 74776 ; http://n2t.net/addgene:74776 ; RRID:Addgene_74776) -
For your References section:
H3K4me3 demethylation by the histone demethylase KDM5C/JARID1C promotes DNA replication origin firing. Rondinelli B, Schwerer H, Antonini E, Gaviraghi M, Lupi A, Frenquelli M, Cittaro D, Segalla S, Lemaitre JM, Tonon G. Nucleic Acids Res. 2015 Mar 11;43(5):2560-74. doi: 10.1093/nar/gkv090. Epub 2015 Feb 23. 10.1093/nar/gkv090 PubMed 25712104