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GST-GINA3
(Plasmid #74979)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74979 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 6031
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rattus norvegicus G protein subunit alpha i3 (Gnai3)
  • Alt name
    GNAI3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1062
  • GenBank ID
    NM_013106.1|
  • Entrez Gene
    Gnai3 (a.k.a. GIP3A)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX-F
  • 3′ sequencing primer pGEX-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-GINA3 was a gift from Pradipta Ghosh (Addgene plasmid # 74979 ; http://n2t.net/addgene:74979 ; RRID:Addgene_74979)
  • For your References section:

    Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin. Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, Kufareva I, Abagyan R, Ghosh P. Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 10.1091/mbc.E14-05-0978. Epub 2014 Sep 3. 10.1091/mbc.E14-05-0978 PubMed 25187647