-
PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cells
-
Depositing Lab
-
Depositing OrganizationUniversity of Texas Southwestern Medical Center
Located in United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75131 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 75131-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 75131-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonep3XFLAG-CMV-14
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 7110
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameinterleukin 1 beta
-
Alt nameIl1b
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)810
-
Mutation(please see depositor comments below)
-
GenBank IDNM_008361
-
Entrez GeneIl1b (a.k.a. IL-1beta, Il-1b)
- Promoter CMV
-
Tag
/ Fusion Protein
- 3XFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation E130K in Il1b was found during Addgene's quality control. The depositor noted that this mutation is expected and does NOT affect its cleavage by caspase 1.
DNA (Catalog # 75131-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 75131-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-pro-Il1b-C-Flag was a gift from Bruce Beutler (Addgene plasmid # 75131 ; http://n2t.net/addgene:75131 ; RRID:Addgene_75131) -
For your References section:
NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, Ulevitch RJ, Beutler B. Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 Dec 7. 10.1038/ni.3333 PubMed 26642356
Map uploaded by the depositor.
