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Human NCKX3-FlagTag (pcDNA3.1-)
(Plasmid #75206)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75206 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1-
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC24A3
  • Alt name
    NCKX3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC24A3 (a.k.a. NCKX3)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG

Cloning Information

Resource Information

Depositor Comments

FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.

Note, in addition to the flag tag, this plasmid differs from Plasmid 75205: Human NCKX3 (pcDNA3.1+) in that it includes a long 3' UTR sequence after the NCX3 ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human NCKX3-FlagTag (pcDNA3.1-) was a gift from Jonathan Lytton (Addgene plasmid # 75206 ; http://n2t.net/addgene:75206 ; RRID:Addgene_75206)
  • For your References section:

    Molecular cloning of a third member of the potassium-dependent sodium-calcium exchanger gene family, NCKX3. Kraev A, Quednau BD, Leach S, Li XF, Dong H, Winkfein R, Perizzolo M, Cai X, Yang R, Philipson KD, Lytton J. J Biol Chem. 2001 Jun 22;276(25):23161-72. Epub 2001 Apr 9. 10.1074/jbc.M102314200 PubMed 11294880