Human NCKX3-FlagTag (pcDNA3.1-)
PurposeMammalian expression of SLC24A3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75206||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSLC24A3 (a.k.a. NCKX3)
- Promoter CMV
/ Fusion Protein
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
Note, in addition to the flag tag, this plasmid differs from Plasmid 75205: Human NCKX3 (pcDNA3.1+) in that it includes a long 3' UTR sequence after the NCX3 ORF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human NCKX3-FlagTag (pcDNA3.1-) was a gift from Jonathan Lytton (Addgene plasmid # 75206 ; http://n2t.net/addgene:75206 ; RRID:Addgene_75206)
For your References section:Molecular cloning of a third member of the potassium-dependent sodium-calcium exchanger gene family, NCKX3. Kraev A, Quednau BD, Leach S, Li XF, Dong H, Winkfein R, Perizzolo M, Cai X, Yang R, Philipson KD, Lytton J. J Biol Chem. 2001 Jun 22;276(25):23161-72. Epub 2001 Apr 9. 10.1074/jbc.M102314200 PubMed 11294880