pmGFPS
(Plasmid #75360)

• Purpose: Fluorescent reporter
• Depositing Lab: Stefan Hohmann
• Publication: monomeric GFP (unpublished)

Backbone

• Vector backbone: YDp-H
• Backbone size w/o insert (bp): 3865
• Total vector size (bp): 4593
• Vector type: integrating vector
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFPmut3
• Alt name: mGFP
• Alt name: monomeric GFP
• Species: Synthetic
• Insert Size (bp): 725
• Mutation: A206K, S65G, S72A
• Promoter: Lac
• Tag / Fusion Protein:
  • HIS3 (from parental YDp-H) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Sma1 (unknown if destroyed)
• 5′ sequencing primer: CTGCAAGGCGATTAAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GFPmut3 sequence was obtained from A. Badrinarayanan, Department of Biochemistry, University of Oxford, UK

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry