PurposeMammalian expression of MBD1v1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78140||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMBD1 (a.k.a. CXXC3, PCM1, RFT)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MBD1v1 pcDNA3.1 was a gift from Heidi Schwarzenbach (Addgene plasmid # 78140 ; http://n2t.net/addgene:78140 ; RRID:Addgene_78140)
For your References section:Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters. Wischnewski F, Friese O, Pantel K, Schwarzenbach H. Mol Cancer Res. 2007 Jul;5(7):749-59. 10.1158/1541-7786.MCR-06-0364 PubMed 17634428