pGEX6p-PBX1
(Plasmid #78145)

• Purpose: bacterial expression of GST-PBX1
• Depositing Lab: Francesco Blasi
• Publication: Mathiasen et al PLoS One. 2015 Apr 9;10(4):e0125789. doi: 10.1371/journal.pone.0125789. eCollection 2015.

Backbone

• Vector backbone: pGEX-6p-2xrbs
• Modifications to backbone: two ribosome binding sites present
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PBX1
• Alt name: pre-B-cell leukemia homeobox 1
• Species: H. sapiens (human)
• Entrez Gene: PBX1 (a.k.a. CAKUHED)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry