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pMJ922
(Plasmid #78312)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78312 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET His6 MBP N10 TEV LIC cloning vector (2C-T)
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 5938
  • Total vector size (bp) 10927
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Alt name
    Cas9
  • Alt name
    Csn1
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4989
  • Mutation
    human codon-optimized gene sequence
  • GenBank ID
    NP_269215
  • Promoter T7
  • Tags / Fusion Proteins
    • His6-MBP-TEV (N terminal on backbone)
    • HA-2xNLS-EGFP-NLS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer MalE FW
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJ922 was a gift from Martin Jinek (Addgene plasmid # 78312 ; http://n2t.net/addgene:78312 ; RRID:Addgene_78312)
  • For your References section:

    Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes. Burger A, Lindsay H, Felker A, Hess C, Anders C, Chiavacci E, Zaugg J, Weber LM, Catena R, Jinek M, Robinson MD, Mosimann C. Development. 2016 Apr 29. pii: dev.134809. 10.1242/dev.134809 PubMed 27130213