pMJ923
(Plasmid #78313)

• Purpose: Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
• Depositing Lab: Martin Jinek
• Publication: Burger et al Development. 2016 Apr 29. pii: dev.134809.

Backbone

• Vector backbone: pET His6 MBP N10 TEV LIC cloning vector (2C-T)
• Backbone manufacturer: Scott Gradia
• Backbone size w/o insert (bp): 5953
• Total vector size (bp): 10927
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SpCas9
• Alt name: Cas9
• Alt name: Csn1
• Species: Streptococcus pyogenes
• Insert Size (bp): 4973
• Mutation: human codon-optimized gene sequence
• GenBank ID: NP_269215
• Promoter: T7
• Tags / Fusion Proteins:
  • His6-MBP-TEV (N terminal on backbone)
  • HA-2xNLS-mCherry-NLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: MalE FW
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry