pJQ200mp19
(Plasmid
#78501)
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Purpose(Empty Backbone) Suicide vector for allelic exchange
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJQ200
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Backbone manufacturerJurgen Quandt
- Backbone size (bp) 4700
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Modifications to backbonepolylinker and lacZ alpha from pUC19 added to pJQ200 as partial PvuI digest.
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Vector typeSuicide vector for gene replacement
- Promoter none
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byConstruct is from our lab.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJQ200mp19 was a gift from Michael Hynes (Addgene plasmid # 78501 ; http://n2t.net/addgene:78501 ; RRID:Addgene_78501) -
For your References section:
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Quandt J, Hynes MF. Gene. 1993 May 15;127(1):15-21. 0378-1119(93)90611-6 [pii] PubMed 8486283