PurposeEncodes zebrafish nm-mhc-b
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78709||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namemyosin, heavy chain 10, non-muscle
SpeciesD. rerio (zebrafish)
Insert Size (bp)580
Entrez Genemyh10 (a.k.a. dZ204D19.2, si:dz150i12.3, wu:fc46h07, wu:fy19d11)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Primers used to amplify cDNA:
CCTGAGCCGAGAGGTCAA and CACGGAGAGCTGCAGGAG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:nm-mhc-b was a gift from Sarah Childs (Addgene plasmid # 78709 ; http://n2t.net/addgene:78709 ; RRID:Addgene_78709)
For your References section:Spatiotemporal expression of smooth muscle markers in developing zebrafish gut. Georgijevic S, Subramanian Y, Rollins EL, Starovic-Subota O, Tang AC, Childs SJ. Dev Dyn. 2007 Jun;236(6):1623-32. 10.1002/dvdy.21165 PubMed 17474123