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pYN4+
(Plasmid #78715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78715 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 78715-D50 50 μg of DNA in Tris buffer $495
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DNA 78715-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pOPRSVICat
  • Backbone manufacturer
    Stratagene
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NHE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    D720G
  • Entrez Gene
    SLC9A1 (a.k.a. APNH, LIKNS, NHE-1, NHE1, PPP1R143)
  • Promoter RSV-LTR
  • Tag / Fusion Protein
    • HA3x (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer TK-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the D720G mutation does not appear to affect protein function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYN4+ was a gift from Larry Fliegel (Addgene plasmid # 78715 ; http://n2t.net/addgene:78715 ; RRID:Addgene_78715)

  • For your References section:

    Structural and functional characterization of transmembrane segment IX of the NHE1 isoform of the Na+/H+ exchanger. Reddy T, Ding J, Li X, Sykes BD, Rainey JK, Fliegel L. J Biol Chem. 2008 Aug 8;283(32):22018-30. doi: 10.1074/jbc.M803447200. Epub 2008 May 28. 10.1074/jbc.M803447200 PubMed 18508767
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