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SQT1665
(Plasmid #78744)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78744 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 78744-D50 50 μg of DNA in Tris buffer $495
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DNA 78744-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAG-GFP
  • Backbone manufacturer
    Cepko lab (Addgene plasmid # 11179)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
  • Alt name
    Cpf1
  • Alt name
    LbCas12a
  • Species
    Lachnospiraceae bacterium ND2006
  • Promoter CAG
  • Tag / Fusion Protein
    • NLS-3xHA (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer M13-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses Cpf1 from Lachnospiraceae bacterium ND2006 in mammalian cells. Recommended to use with BPK3082 (Addgene plasmid# 78742), which can be used to express a gRNA for LbCpf1.

Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SQT1665 was a gift from Keith Joung (Addgene plasmid # 78744 ; http://n2t.net/addgene:78744 ; RRID:Addgene_78744)

  • For your References section:

    Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. 10.1038/nbt.3620 PubMed 27347757
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