PurposeTo overexpress p14ARF in Mammalian Cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78764||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerderived from pCS2+
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCDKN2A (a.k.a. ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
- Promoter CMV IE94
/ Fusion Protein
- 3xHA (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pCS4+ is based on pCS2+, with the following unique restriction endonuclease sites are added to MCS: BsrG1, EcoRV, AscI, NcoI, EcoO019, Sfi I, Fes I, Bgl II, Nhe I. A start codon with the Kozak sequence and stop codons in all three frames are added. pCS5+ is the same as pCS4+ except that the tag is Cterminal.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS5-3HA/p14ARF was a gift from Jaewhan Song (Addgene plasmid # 78764 ; http://n2t.net/addgene:78764 ; RRID:Addgene_78764)
For your References section:Acceleration of gastric tumorigenesis through MKRN1-mediated posttranslational regulation of p14ARF. Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong JS, Chun KH, Song J. J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 10.1093/jnci/djs424. Epub 2012 Oct 25. 10.1093/jnci/djs424 PubMed 23104211