PCS4 3XFLAG-PPARgamma2
(Plasmid #78770)

• Purpose: To overexpress PPARgamma2 in Mammalian Cells
• Depositing Lab: Jaewhan Song
• Publication: Kim et al Cell Death Differ. 2014 Apr;21(4):594-603. doi: 10.1038/cdd.2013.181. Epub 2013 Dec 13.

Backbone

• Vector backbone: pCS4-3FLAG
• Backbone manufacturer: derived from pCS2+
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR_2
• Species: M. musculus (mouse)
• Entrez Gene: Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCS4+ is based on pCS2+, with the following unique restriction endonuclease sites are added to MCS: BsrG1, EcoRV, AscI, NcoI, EcoO019, Sfi I, Fes I, Bgl II, Nhe I. A start codon with the Kozak sequence and stop codons in all three frames are added.