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CC1215
(Bacterial strain #79062)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 79062 Bacteria in agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    CC1215 (purE)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    none
  • Promoter none

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ODN 1161: 5'-CGGATGACGTTGAGCTGATTCATTTTCC
  • 3′ sequencing primer ODN 1164: 5'-CAGCTCACCTAAACGATCAAACAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genotype = pur
Precursor strain = BL21(DE3)

This strain was built by Kelly L. Sullivan in the lab of T. J. Kappock. It is used to produce PurE proteins, including low-activity mutants, in a zero-background host lacking a functional purE1 gene. Both N5-CAIR mutase (purE1; EC 5.4.99.18) and AIR carboxylase (purE2; EC 4.1.1.21) mutants have been successfully overproduced in this strain.

Purine auxotrophy results from a defect in the de novo biosynthesis of purine nucleotides. The auxotrophy can be functionally complemented with adenine, hypoxanthine, or other substrate for the purine salvage pathway. There is no evident requirement for His or thiamine.

Strains lacking a functional purE1 gene excrete AIR nucleoside (AIRs) which spontaneously forms a brown polymer. Culture media and plates may have a darker pigmentation unless a functional purE (either purE1 or purE2) is present or a purine base, which represses production of de novo biosynthesis enzymes, is supplied.

The deletion cassette ΔpurE736::kan from Keio strain JW0512 was transduced (using P1) to disrupt the target gene in BL21(DE3). Subsequent attempts to excise the cassette were unsuccessful.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CC1215 was a gift from T. J. Kappock (Addgene plasmid # 79062)