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pEF6-myc-TBC1D15
(Plasmid #79148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79148 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 79148-D50 50 μg of DNA in Tris buffer $495
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DNA 79148-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEF6/V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TBC1D15
  • Alt name
    TBC1 domain family, member 15
  • Species
    M. musculus (mouse)
  • Mutation
    S616N (please see depositor comment below)
  • Entrez Gene
    Tbc1d15 (a.k.a. 4432405K22Rik, AU022358, AU023730, AW557774, Ly6dl, Rab7-GAP)
  • Promoter EF-1alpha
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • V5 (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note- The TBC1D15 mutation S616N identified during Addgene's quality control process does not affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6-myc-TBC1D15 was a gift from Aimee Edinger (Addgene plasmid # 79148 ; http://n2t.net/addgene:79148 ; RRID:Addgene_79148)

  • For your References section:

    Differential effects of TBC1D15 and mammalian Vps39 on Rab7 activation state, lysosomal morphology, and growth factor dependence. Peralta ER, Martin BC, Edinger AL. J Biol Chem. 2010 May 28. 285(22):16814-21. 10.1074/jbc.M110.111633 PubMed 20363736
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