RF-211-UB-Lyn
(Plasmid #79184)

• Purpose: Expression of the C-terminal fragment of Lyn kinase that is endogenously generated by caspase cleavage in mammalian cells
• Depositing Lab: Richard Fahlman
• Publication: Eldeeb et al Oncotarget. 2014 May 15;5(9):2714-22.

Backbone

• Vector backbone: pcDNA 3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lyn
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: delta 1-18
• Entrez Gene: LYN (a.k.a. JTK8, SAIDV, p53Lyn, p56Lyn)
• Promoter: CMV
• Tags / Fusion Proteins:
  • ubiquitin (N terminal on backbone)
  • 3x Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry