pDGE6
(Plasmid
#79476)
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PurposesgRNA shuttle vector; module 1E
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Depositing Lab
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Depositing OrganizationMartin-Luther-Universitaet Halle-Wittenberg, Faculty of Medicine
Located in Germany -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 79476-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 79476-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC57
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Modifications to backboneBsaI site (in bla) eliminated
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Vector typeCRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAtU6-ccdB-sgRNA
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gRNA/shRNA sequencenone, empty sgRNA shuttle vector
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Insert Size (bp)1498
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MutationBsaI site eliminated
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Entrez GeneccdB(letD) (a.k.a. D616_p97105, Fpla045)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 79476-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 79476-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDGE6 was a gift from Johannes Stuttmann (Addgene plasmid # 79476 ; http://n2t.net/addgene:79476 ; RRID:Addgene_79476) -
For your References section:
Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. 10.1111/tpj.13319 PubMed 27579989
Map uploaded by the depositor.
