Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
79440pDGE1recipient plasmid [multiplexing]; p35S:Cas9, nptII
79441pDGE2recipient plasmid [multiplexing]; p35S:Cas9, BASTA
79442pDGE3recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII
79443pDGE4recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA
79444pDGE62recipient plasmid [single nuclease]; p35S:Cas9, nptII
79445pDGE63recipient plasmid [single nuclease]; p35S:Cas9, BASTA
79446pDGE64recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII
79447pDGE65recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA
79448pDGE144recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA
79449pDGE146recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA
79450pDGE148recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA
79451pDGE151recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA
79452pDGE160recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII
79453pDGE161recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptII
79454pDGE164recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII
79455pDGE165recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII
79456pDGE166recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro
79457pDGE167recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro
79458pDGE168recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro
79459pDGE169recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro
79461pDGE5sgRNA shuttle vector; module 1
79462pDGE7sgRNA shuttle vector; module 2
79463pDGE8sgRNA shuttle vector; module 2E
79465pDGE9sgRNA shuttle vector; module 3
79466pDGE10sgRNA shuttle vector; module 4
79467pDGE11sgRNA shuttle vector; module 4E
79469pDGE12sgRNA shuttle vector; module 5
79471pDGE13sgRNA shuttle vector; module 6
79473pDGE14sgRNA shuttle vector; module 7
79474pDGE15sgRNA shuttle vector; module 8E
79476pDGE6sgRNA shuttle vector; module 1E
80578pDGE76recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII
80579pDGE77recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA
80580pDGE78recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII
80581pDGE79recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA
80582pJOG250recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII
80583pJOG251recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
80584pJOG252recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII
80585pJOG253recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
80586pJOG292empty vector (lacZ) for 2nd generation recipient assembly
80587pJOG294dummy module (ccdB) for 2nd generation recipient assembly

Antibodies from Article