Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #79541)


Item Catalog # Description Quantity Price (USD)
Plasmid 79541 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Addgene Plasmid #44456
  • Total vector size (bp) 9435
  • Modifications to backbone
    Replaced CamR with KanR.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Tags / Fusion Proteins
    • His tag (N terminal on insert)
    • LAA degradation tag (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name

Gene/Insert 3

  • Gene/Insert name

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid is derived from the Dual-Recombinase-Controller (addgene plasmid 44456) from the Endy Lab, modified to have KanR instead of CamR on the backbone, and also with and added recombinase (A118) and inducible promoter system (PhlF) to drive it.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The DH5alphaPRO strain is DH5alpha modified to express LacI and TetR constitutively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNR220 was a gift from Timothy Lu (Addgene plasmid # 79541 ; ; RRID:Addgene_79541)
  • For your References section:

    Synthetic recombinase-based state machines in living cells. Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK. Science. 2016 Jul 22;353(6297):aad8559. doi: 10.1126/science.aad8559. Epub 2016 Jul 21. 10.1126/science.aad8559 PubMed 27463678