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Synthetic recombinase-based state machines in living cells.
Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
Science. 2016 Jul 22;353(6297):aad8559. doi: 10.1126/science.aad8559. Epub 2016 Jul 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79540pNR64Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
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79541pNR220Input plasmid: expresses three serine recombinases downstream of three inducible promoters.
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79542pNR160Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.
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79543pNR188Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.
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79544pNR163Output plasmid: contains 5-state register that regulates expression of GFP.
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79545pNR164Output plasmid: contains 5-state register that regulates expression of GFP.
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79546pNR230BxbI attB-attP. GFP will express if sites recombine.
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79547pNR166Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.
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79548pNR292Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.
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79550pNR284Output plasmid: contains 16-state register that regulates expression of GFP.
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79552pNR186Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.
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79553pNR187Output plasmid: contains 5-state register that regulates expression of GFP.
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79555pNR291Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
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79576pNR239A118 attB-attP. GFP will express if sites recombine.
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79577pNR276TP901 attB-attP. GFP will express if sites recombine.
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79578pNR279BxbI attL-attR. GFP expression will stop if sites recombine.
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79579pNR280A118 attL-attR. GFP expression will stop if sites recombine.
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79580pNR287TP901 attL-attR. GFP expression will stop if sites recombine.
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79646pNR165Output plasmid: contains 5-state register that regulates expression of GFP.
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