MO70-hJunctate
(Plasmid #79596)

• Purpose: Transient and retroviral expression
• Depositing Lab: Yousang Gwack
• Publication: Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;109(22):8682-7. doi: 10.1073/pnas.1200667109. Epub 2012 May 14.

Backbone

• Vector backbone: MO70
• Backbone size w/o insert (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: junctate
• Alt name: ASPH
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Entrez Gene: ASPH (a.k.a. AAH, BAH, CASQ2BP1, FDLAB, HAAH, JCTN, junctin)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference. The Junctate splice variant contained in this plasmid does not have the 15 extra amino acids present in GenBank reference sequence NM_032468.4. Please see Figure S1b in the Supporting Information section of the associated publication for more details and the entire amino acid sequence of the clone. This clone was numbered following the human cDNA clone AF306765.