PurposeTransient and retroviral expression
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79596||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7480
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)900
Entrez GeneASPH (a.k.a. AAH, BAH, CASQ2BP1, FDLAB, HAAH, JCTN, junctin)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-Rev (Common Sequencing Primers)
Terms and Licenses
This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference. The Junctate splice variant contained in this plasmid does not have the 15 extra amino acids present in GenBank reference sequence NM_032468.4. Please see Figure S1b in the Supporting Information section of the associated publication for more details and the entire amino acid sequence of the clone. This clone was numbered following the human cDNA clone AF306765.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MO70-hJunctate was a gift from Yousang Gwack (Addgene plasmid # 79596 ; http://n2t.net/addgene:79596 ; RRID:Addgene_79596)
For your References section:Junctate is a Ca2+-sensing structural component of Orai1 and stromal interaction molecule 1 (STIM1). Srikanth S, Jew M, Kim KD, Yee MK, Abramson J, Gwack Y. Proc Natl Acad Sci U S A. 2012 May 29;109(22):8682-7. doi: 10.1073/pnas.1200667109. Epub 2012 May 14. 10.1073/pnas.1200667109 PubMed 22586105