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MO70-hJunctate - EF-mutant
(Plasmid #79597)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79597 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MO70
  • Backbone size w/o insert (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    junctate
  • Alt name
    ASPH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    77DAD79 to 77AAA79
  • Entrez Gene
    ASPH (a.k.a. AAH, BAH, CASQ2BP1, FDLAB, HAAH, JCTN, junctin)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference. Please see Figure S1b in the Supporting Information section of the associated publication for more details regarding the EF-hand mutation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MO70-hJunctate - EF-mutant was a gift from Yousang Gwack (Addgene plasmid # 79597 ; http://n2t.net/addgene:79597 ; RRID:Addgene_79597)
  • For your References section:

    Junctate is a Ca2+-sensing structural component of Orai1 and stromal interaction molecule 1 (STIM1). Srikanth S, Jew M, Kim KD, Yee MK, Abramson J, Gwack Y. Proc Natl Acad Sci U S A. 2012 May 29;109(22):8682-7. doi: 10.1073/pnas.1200667109. Epub 2012 May 14. 10.1073/pnas.1200667109 PubMed 22586105