MO70-hJunctate - EF-mutant
(Plasmid #79597)

• Purpose: Transient and retroviral expression
• Depositing Lab: Yousang Gwack
• Publication: Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;109(22):8682-7. doi: 10.1073/pnas.1200667109. Epub 2012 May 14.

Backbone

• Vector backbone: MO70
• Backbone size w/o insert (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: junctate
• Alt name: ASPH
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: 77DAD79 to 77AAA79
• Entrez Gene: ASPH (a.k.a. AAH, BAH, CASQ2BP1, FDLAB, HAAH, JCTN, junctin)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference. Please see Figure S1b in the Supporting Information section of the associated publication for more details regarding the EF-hand mutation.