FRT_htr2c(c)-GFP
(Plasmid #79677)

• Purpose: serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP
• Depositing Lab: Stefan Stamm
• Publication: Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. doi: 10.15252/emmm.201506030.

Backbone

• Vector backbone: pcDNA5_FTR
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 11000
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: serotonin receptor 2C
• Alt name: 5HT2C
• Species: H. sapiens (human)
• Insert Size (bp): 6000
• Mutation: splice site of exon Vb in consensus
• Entrez Gene: HTR2C (a.k.a. 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP in Frame (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry