pUNIV-SIG-5HTR4D100A
(Plasmid #16312)

• Depositing Lab: Bruce Conklin
• Depositing Organization: J. David Gladstone Institutes
  Located in the United States of America
• Publication: Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 105(4):1209-14.

Backbone

• Vector backbone: pUNIV
• Backbone size w/o insert (bp): 5257
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: h5HT4b Serotonin Receptor (Rs1 D100A RASSL)
• Alt name: Rs1
• Alt name: 5HT4
• Alt name: HTR4
• Species: H. sapiens (human)
• Insert Size (bp): 1272
• Mutation: Added FLAG tag; Aspartic Acid 100 changed to Alanine.
• Entrez Gene: HTR4 (a.k.a. 5-HT4, 5-HT4R)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid backbone is from Drs. Wes Kroeze and Bryan Roth (Univ. of North Carolina, Chapel Hill, NC)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 16312-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 16312-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.