5HT2C-RNA2-Flag
(Plasmid
#79679)
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Purposeserotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79679 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerclonetech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5900
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameserotonin receptor 2C, RNA2
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Alt name5HT2C
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Entrez GeneHTR2C (a.k.a. 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamH1 (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5HT2C-RNA2-Flag was a gift from Stefan Stamm (Addgene plasmid # 79679 ; http://n2t.net/addgene:79679 ; RRID:Addgene_79679) -
For your References section:
Oligonucleotide-induced alternative splicing of serotonin 2C receptor reduces food intake. Zhang Z, Shen M, Gresch PJ, Ghamari-Langroudi M, Rabchevsky AG, Emeson RB, Stamm S. EMBO Mol Med. 2016 Aug 1;8(8):878-94. doi: 10.15252/emmm.201506030. 10.15252/emmm.201506030 PubMed 27406820
Map uploaded by the depositor.
