PurposemiRFP PAS domain fused to FRB
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80002||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerARIAD Pharmaceuticals, Inc.
- Backbone size w/o insert (bp) 1910
- Total vector size (bp) 2604
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Insert Size (bp)694
- Promoter CMV
/ Fusion Protein
- PAS domain of miRFP (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer ctcttcggccagcgaattc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC4-RHE-PAS (FRB-PAS) was a gift from Vladislav Verkhusha (Addgene plasmid # 80002 ; http://n2t.net/addgene:80002 ; RRID:Addgene_80002)
For your References section:Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV. Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. 10.1038/ncomms12405 PubMed 27539380