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PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 80070-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 80070-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneEGFP-C1
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Backbone manufacturerCLONTECH
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 9112
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Modifications to backboneSalI cloning site duplicated. No other changes
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBLM
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SpeciesH. sapiens (human)
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MutationIf correct ID, the cDNA is from HeLa and has characteristic African-ancestry nt
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GenBank IDU39817.1 NP_000048.1
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Entrez GeneBLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer not sure
- 3′ sequencing primer not sure
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 80070-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 80070-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-BLM was a gift from Nathan Ellis (Addgene plasmid # 80070 ; http://n2t.net/addgene:80070 ; RRID:Addgene_80070) -
For your References section:
Evidence for BLM and Topoisomerase IIIalpha interaction in genomic stability. Hu P, Beresten SF, van Brabant AJ, Ye TZ, Pandolfi PP, Johnson FB, Guarente L, Ellis NA. Hum Mol Genet. 2001 Jun 1;10(12):1287-98. PubMed 11406610
Map uploaded by the depositor.
