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pWUKI Cas1 Cas2 T72I
(Plasmid #80100)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80100 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas1
  • Entrez Gene
    ygbT (a.k.a. b2755, ECK2750, JW2725, cas1)

Gene/Insert 2

  • Gene/Insert name
    Cas2
  • Mutation
    T72I
  • Entrez Gene
    ygbF (a.k.a. b2754, ECK2749, JW5438, cas2)

Gene/Insert 3

  • Gene/Insert name
    CRISPR Array

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWUKI Cas1 Cas2 T72I was a gift from George Church (Addgene plasmid # 80100)
  • For your References section:

    Molecular recordings by directed CRISPR spacer acquisition. Shipman SL, Nivala J, Macklis JD, Church GM. Science. 2016 Jun 9. pii: aaf1175. 10.1126/science.aaf1175 PubMed 27284167