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Molecular recordings by directed CRISPR spacer acquisition.
Shipman SL, Nivala J, Macklis JD, Church GM
Science. 2016 Jun 9. pii: aaf1175.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79879pWUKI 1+2Encodes Cas1+2 and a CRISPR array
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80088pRSF-DUET Cas1 Cas2Encodes Cas1 and Cas2
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80089pWURA Cas1Encodes Cas1
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80090pWUR Cas2Encodes Cas2
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80091pWUKI Cas1 Cas2 E9QEncodes Cas1 and Cas2 E9Q
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80092pWUKI Cas1 V134L Cas2Encodes Cas1 V134L and Cas2
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80093pWUKI Cas1 V33A Cas2Encodes Cas1 V33A and Cas2
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80094pWUKI Cas1 V190I Cas2Encodes Cas1 V190I and Cas2
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80095pWUKI Cas1 P279L Cas2Encodes Cas1 P279L and Cas2
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80096pWUKI Cas1 P202Q Cas2Encodes Cas1 P202Q and Cas2
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80097pWUKI Cas1 P212L Cas2Encodes Cas1 P212L and Cas2
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80098pWUKI Cas1 P282L Cas2Encodes Cas1 P282L and Cas2
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80099pWUKI Cas1 P294H Cas2Encodes Cas1 P294H and Cas2
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80100pWUKI Cas1 Cas2 T72IEncodes Cas1 and Cas2 T72I
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80101pWUKI Cas1 Cas2 G74AEncodes Cas1 and Cas2 G74A
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80102pWUR 1+2 tetO mut89Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2
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Antibodies from Article