PurposeExpresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80592||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneAAV ITR backbone
- Backbone size w/o insert (bp) 5207
- Total vector size (bp) 5957
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)750
MutationInserted 30 nt hairpin which is targeted by Csy4 immediately following the start codon.
- Promoter CBA
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer caaatctgtgcggagccgaaatctgggagg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TR- ATG-HP-GFP was a gift from Aravind Asokan (Addgene plasmid # 80592 ; http://n2t.net/addgene:80592 ; RRID:Addgene_80592)
For your References section:Controlling mRNA stability and translation with the CRISPR endoribonuclease Csy4. Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A. RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015 Sep 9. 10.1261/rna.051227.115 PubMed 26354771