Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

GTH-r
(Plasmid #81106)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 81106 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSIVh
  • Total vector size (bp) 4293
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Alt name
    mCherry
  • Insert Size (bp)
    750
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GTH-r was a gift from Serge Pelet (Addgene plasmid # 81106 ; http://n2t.net/addgene:81106 ; RRID:Addgene_81106)
  • For your References section:

    New families of single integration vectors and gene tagging plasmids for genetic manipulations in budding yeast. Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S. Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16. 10.1007/s00438-016-1249-1 [pii] PubMed 27637489