Purposean updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||82022||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6260
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter Pcptoo
- Cloning method Gibson Cloning
- 5′ sequencing primer GCTTCCGGCTCGTATGTTGTG
- 3′ sequencing primer TTGGGTAACGCCAGGGT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pALM174 was a gift from Brian Pfleger (Addgene plasmid # 82022 ; http://n2t.net/addgene:82022 ; RRID:Addgene_82022)
For your References section:Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF. ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. Epub 2014 Sep 25. 10.1021/sb500260k PubMed 25216157