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Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. Epub 2014 Sep 25. PubMed Article

Plasmids from Article

ID Plasmid Purpose
82019pACSA_Pcpt_YFPpALM34; control for YFP expression from cyanobacterial phycocyanin truncated promoter
82020pACSA_PcptOO_YFP_PMB2__LacIWFcLac94; pALM94; IPTG inducible system
82021pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWFcLac143; pALM143; IPTG inducible system
82022pALM174an updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression
82023pALM179an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

Antibodies from Article