pLenti-V5-KDM3A-mut
(Plasmid #82332)

• Purpose: lentiviral expression of demethylase-inactive KDM3A H1120G/D1122N
• Depositing Lab: Luke Gaughan
• Publication: Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-207. doi: 10.1093/nar/gku1298. Epub 2014 Dec 8.

Backbone

• Vector backbone: pLenti6
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KDM3A
• Species: H. sapiens (human)
• Mutation: demethylase-inactive H1120G/D1122N and two silent mutations to make it siRNA resistant
• Entrez Gene: KDM3A (a.k.a. JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry