PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||82407||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBD Biosciences Clontech
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Promoter CMV
/ Fusion Protein
- leader sequence of the E1α subunit of pyruvate dehydrogenase for mitochondrial targeting (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-N1-mt-ro1GFP was a gift from S. James Remington (Addgene plasmid # 82407 ; http://n2t.net/addgene:82407 ; RRID:Addgene_82407)
For your References section:Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, Remington SJ. J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13. 10.1074/jbc.M312846200 PubMed 14722062