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pEGFP-N1-mt-ro1GFP
(Plasmid #82407)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82407 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    BD Biosciences Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ro1GFP
  • Species
    jellyfish
  • Mutation
    C48S/F64L/T65S/S147C/Q204C
  • Promoter CMV
  • Tag / Fusion Protein
    • leader sequence of the E1α subunit of pyruvate dehydrogenase for mitochondrial targeting (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-mt-ro1GFP was a gift from S. James Remington (Addgene plasmid # 82407 ; http://n2t.net/addgene:82407 ; RRID:Addgene_82407)
  • For your References section:

    Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, Remington SJ. J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13. 10.1074/jbc.M312846200 PubMed 14722062