pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag
(Plasmid #82469)

• Purpose: Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
• Depositing Lab: Daniel Schoenberg
• Publication: Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. Epub 2009 Feb 17.

Backbone

• Vector backbone: pcDNA4-T/O
• Backbone size w/o insert (bp): 5008
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRNA capping enzyme
• Species: M. musculus (mouse)
• Insert Size (bp): 1911
• Mutation: changed Lysine 294 to Alanine
• Entrez Gene: Rngtt (a.k.a. AU020997, HCE, MCE1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Apa1 (not destroyed)
• 5′ sequencing primer: CMV primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry